Sare Asli, Ramiz Eid & Muhamad Hugerat
To cite this article: Sare Asli, Ramiz Eid & Muhamad Hugerat (2021): A novel pretreatment
biotechnology for increasing methane yield from lipid-rich wastewater based on combination of
hydrolytic enzymes with Candidarugosa fungus, Preparative Biochemistry & Biotechnology, DOI:
10.1080/10826068.2021.1901233
To link to this article: https://doi.org/10.1080/10826068.2021.1901233
ABSTRACT
Lipid-rich wastewater from the local dairy industry (cheese whey) in the Galilee, Israel was hydrolyzed by using two different sources of lipase as hydrolytic enzymes: fungal (Candida rogusa lipase-AY) and animal porcine pancreatic lipase(PPL). Pretreatment efficiency was verified by
comparative biodegradability tests of raw and treated wastewater samples. Simultaneous hydrolysis and anaerobic digestion in the same reactors were also tested. Enzymatic pretreatment of
these samples at a concentration of 0.05 w v1 showed organic matter removal of 90% and
methane formation increases of 140% for the fungal source enzyme (i.e., AY), while for the animal
source enzyme (i.e., PPL) was 86 and 130%, respectively. Enzymatic pretreatment led to significant
methane formation which was obtained only for moderate substrate concentration (initial chemical oxygen demand of 15 gL1
); While in high concentrated lipid-rich wastewater led to methane
yield inhibition. The main finding was that the combination of AY enzyme with Candida rugosa
fungus (i.e., enzyme mixture) led to a high efficiency in methane production (þ152%) and organic
materials removal (more than 90%). In summary, the use of fungal hydrolytic lipase mixed with
Candida rugosa fungus is a promising method for enhancing methane production during the biodegradation of fat and grease-rich wastewater
